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anti cox2  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology anti cox2
    Anti Cox2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cox2/MTCO2+Rabbit+mAb/pm42849804-72-42-44
    Average 93 stars, based on 5 article reviews
    anti cox2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Phytohormone Strigolactams Overcomes Oxaliplatin Resistance in Colorectal Cancer by Blocking the Late-Stage Autophagy to Tip the Metabolic Balance toward Ferroptosis.
    Article Snippet: Primary and acquired oxaliplatin (OXA)-resistance remains a major challenge in colorectal cancer (CRC) therapy.. In this work, the type-II strigolactam SL 39 was identified as a promising lead compound against OXA-resistant HCT116R cells.. Co-treatment of SL 39 with OXA restored its sensitivity to HCT116R cells and inhibited xenograft tumor growth.

    Article Title: Astaxanthin suppress ferroptosis through the Akt1-FoxO3a signaling pathway to alleviates brain injury after intracerebral hemorrhage.
    Article Snippet: Following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Servicebio, Wuhan, China), proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, USA). .. Membranes were blocked with 5 % skim milk at room temperature for 2 h. Subsequently, membranes were incubated overnight at 4 ◦C with the following primary antibodies: GPX4 (67763-1-Ig; 1:2000; Proteintech Group, China), SLC7A11 (26864-1-AP; 1:2000; Proteintech Group, China), phosphorylated Akt1 (P-Akt1; 80462-1-RR; 1:4000; Proteintech Group, China), total Akt1 (T-Akt1; 80816-1-RR; 1:10000; Proteintech Group, China), phosphorylated FoxO3a (P-FoxO3a; 28755-1-AP; 1:1000; Proteintech Group, China), total FoxO3a (T-FoxO3a; 10849-1- AP; 1:5000; Proteintech Group, China), GAPDH (60004-1-AP; 1:100000; Proteintech Group, China), COX2 (A1253; 1:1000; ABclonal, China), IL-1β (A20529; 1:1000; ABclonal, China), and IL-6 (A0286; 1:1000; ABclonal, China). ..

    Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics.
    Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), COX2 (1:1500, Abclonal, A1253), FTH1 (1:1500, Abclonal, A19544), Drp1 (1:2000, Abcam, ab184247), OPA1 (1:2000, Proteintech, 27733–1-AP), MFN1 (1:2000, Novusbio, NBP1–51841), MFN2 (1:4000, Abcam, ab124773). ..

    Western Blot:

    Article Title: Manganese-loaded carbon nanoparticles ameliorate ANCA-associated vasculitis by inhibiting ferroptosis.
    Article Snippet: .. Western blotting (WB) was performed using specific primary antibodies against TFR1, manganese superoxide dismutase (MnSOD) (ABclonal, China), COX2 (Wanlei, China), CPX4 (Abcam), ICAM-1 (Proteintech, China), VCAM-1 (Boster, China) and GAPDH (Abways, China. ..

    SDS Page:

    Article Title: Nicotinamide mononucleotide combined glucose counteracts iron overload induced mitochondrial dysfunction in mice under hypobaric hypoxia.
    Article Snippet: .. SDS-PAGE separated the proteins, transferred them onto a nitrocellulose membrane, and probed with specific antibodies against SLC7A11 (ABclonal, A13685), FSP1 (ABclonal, A12128), GPX4 (ProteinTech, 67763-1-Ig), COX1, (ABclonal, A7531), COX2, (ABclonal, A1253), SDHA (ABclonal, A2594), β-ACTIN as an internal standard. ..

    Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics.
    Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), COX2 (1:1500, Abclonal, A1253), FTH1 (1:1500, Abclonal, A19544), Drp1 (1:2000, Abcam, ab184247), OPA1 (1:2000, Proteintech, 27733–1-AP), MFN1 (1:2000, Novusbio, NBP1–51841), MFN2 (1:4000, Abcam, ab124773). ..

    Membrane:

    Article Title: Nicotinamide mononucleotide combined glucose counteracts iron overload induced mitochondrial dysfunction in mice under hypobaric hypoxia.
    Article Snippet: .. SDS-PAGE separated the proteins, transferred them onto a nitrocellulose membrane, and probed with specific antibodies against SLC7A11 (ABclonal, A13685), FSP1 (ABclonal, A12128), GPX4 (ProteinTech, 67763-1-Ig), COX1, (ABclonal, A7531), COX2, (ABclonal, A1253), SDHA (ABclonal, A2594), β-ACTIN as an internal standard. ..

    Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics.
    Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), COX2 (1:1500, Abclonal, A1253), FTH1 (1:1500, Abclonal, A19544), Drp1 (1:2000, Abcam, ab184247), OPA1 (1:2000, Proteintech, 27733–1-AP), MFN1 (1:2000, Novusbio, NBP1–51841), MFN2 (1:4000, Abcam, ab124773). ..

    Lysis:

    Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics.
    Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), COX2 (1:1500, Abclonal, A1253), FTH1 (1:1500, Abclonal, A19544), Drp1 (1:2000, Abcam, ab184247), OPA1 (1:2000, Proteintech, 27733–1-AP), MFN1 (1:2000, Novusbio, NBP1–51841), MFN2 (1:4000, Abcam, ab124773). ..

    Nucleic Acid Electrophoresis:

    Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics.
    Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), COX2 (1:1500, Abclonal, A1253), FTH1 (1:1500, Abclonal, A19544), Drp1 (1:2000, Abcam, ab184247), OPA1 (1:2000, Proteintech, 27733–1-AP), MFN1 (1:2000, Novusbio, NBP1–51841), MFN2 (1:4000, Abcam, ab124773). ..

    Pyrolysis Gas Chromatography:

    Article Title: Periplocin and bufalin induce cardiotoxicity by regulating AMPK/SIRT1/PGC-1α pathway to inhibit energy metabolism and trigger autophagy.
    Article Snippet: Objective: Periplocin (PE) and bufalin (BU)—active ingredients of traditional Chinese medicine—exhibit diverse biological activities, including cardiotonic and antitumor effects.. However, their clinical application is limited by dose-dependent cardiotoxicity, the specific mechanisms of which remain unclear.. Methods: The cardiotoxicity of PE and BU was evaluated using cardiomyoblasts and zebrafish as models.

    Ubiquitin Proteomics:

    Article Title: Periplocin and bufalin induce cardiotoxicity by regulating AMPK/SIRT1/PGC-1α pathway to inhibit energy metabolism and trigger autophagy.
    Article Snippet: Objective: Periplocin (PE) and bufalin (BU)—active ingredients of traditional Chinese medicine—exhibit diverse biological activities, including cardiotonic and antitumor effects.. However, their clinical application is limited by dose-dependent cardiotoxicity, the specific mechanisms of which remain unclear.. Methods: The cardiotoxicity of PE and BU was evaluated using cardiomyoblasts and zebrafish as models.

    other:

    Article Title: A colon-targeted nanozyme-inulin hydrogel system for reactive oxygen species scavenging and gut microbiota modulation in inflammatory bowel disease therapy.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of <t>COX‐2,</t> TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.
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    Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of <t>COX‐2,</t> TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.
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    Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of <t>COX‐2,</t> TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.
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    Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of <t>COX‐2,</t> TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.
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    Upregulation of proteins associated with uterine TLR2/4–NF-κB–COX-2 inflammatory signaling in rats with Gardnerella vaginalis -associated bacterial vaginosis. (A) Relative protein abundance of Toll-like receptor 2 (TLR2), TLR4, phospho-NF-κB p65 at serine 536 (pS536-p65) <t>and</t> <t>cyclooxygenase-2</t> (COX-2) in uterine tissues. Data are presented as the means ( X ¯ ) ± standard error of the mean (SEM); n = 3 independent uterine tissue samples per group. Statistical significance was determined using Student’s t-tests . Complete densitometric values are provided in <xref ref-type=Supplementary Table 11 . (B) Representative Western blot images. Protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Original uncropped Western blot images are provided in Supplementary Figure 1 . " width="250" height="auto" />
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    Upregulation of proteins associated with uterine TLR2/4–NF-κB–COX-2 inflammatory signaling in rats with Gardnerella vaginalis -associated bacterial vaginosis. (A) Relative protein abundance of Toll-like receptor 2 (TLR2), TLR4, phospho-NF-κB p65 at serine 536 (pS536-p65) <t>and</t> <t>cyclooxygenase-2</t> (COX-2) in uterine tissues. Data are presented as the means ( X ¯ ) ± standard error of the mean (SEM); n = 3 independent uterine tissue samples per group. Statistical significance was determined using Student’s t-tests . Complete densitometric values are provided in <xref ref-type=Supplementary Table 11 . (B) Representative Western blot images. Protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Original uncropped Western blot images are provided in Supplementary Figure 1 . " width="250" height="auto" />
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    Image Search Results


    Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of COX‐2, TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.

    Journal: Exploration

    Article Title: Soluble Nanomedicine Against Soluble Neurosensitizers to Effectively Relieve Osteoarthritis Pain and Joint Damage

    doi: 10.1002/exp2.70233

    Figure Lengend Snippet: Therapeutic effect of LAC in the monoiodoacetate mouse model. (A) H&E staining of the joint from MIA mice after different treatments. (B) Quantitative analysis of the synovitis score. (C) Representative images of SO&FG‐stained knee joint sections from MIA mice with different treatments. (D) The severity of OA‐like lesions was analyzed using the OARSI scoring system. (E) Immunofluorescence imaging of iNOS (red)/CD206 (green) within the knee joints of mice with different treatments. (F) Corresponding quantification of the MFI of CD206 in the synovium of mice with different treatments. (G) Immunohistochemical staining of COX‐2, TNF‐α, and TRAP in the knee joints of mice with different treatments. (H,I) Quantification of the relative density of COX‐2 + (H) and TNF‐α + (I) cells in the knee joints of mice with different treatments. n = 5 in B and D, and n = 3 in F, H, and I, mean ± SD. The significant differences are determined by one‐way ANOVA analysis with Tukey's multiple comparison test. ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns stands for no significant difference.

    Article Snippet: Proteins in equivalent amounts were then separated on a 10% sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to the PVDF membrane, followed by blocking in TBS‐T solution containing 5% skim milk for 1 h. Subsequently, the membrane was incubated with primary antibody against COX‐2 (66351‐1‐Ig, Proteintech), iNOS (80517‐1‐RR, Proteintech), and CD206 (60143‐1‐Ig, Proteintech) overnight at 4°C.

    Techniques: Staining, Analysis, Immunofluorescence, Imaging, Immunohistochemical staining, Comparison

    Upregulation of proteins associated with uterine TLR2/4–NF-κB–COX-2 inflammatory signaling in rats with Gardnerella vaginalis -associated bacterial vaginosis. (A) Relative protein abundance of Toll-like receptor 2 (TLR2), TLR4, phospho-NF-κB p65 at serine 536 (pS536-p65) and cyclooxygenase-2 (COX-2) in uterine tissues. Data are presented as the means ( X ¯ ) ± standard error of the mean (SEM); n = 3 independent uterine tissue samples per group. Statistical significance was determined using Student’s t-tests . Complete densitometric values are provided in <xref ref-type=Supplementary Table 11 . (B) Representative Western blot images. Protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Original uncropped Western blot images are provided in Supplementary Figure 1 . " width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: Inflammatory landscape of Gardnerella vaginalis -associated bacterial vaginosis in rats: an integrated multi-omics analysis

    doi: 10.3389/fimmu.2026.1914434

    Figure Lengend Snippet: Upregulation of proteins associated with uterine TLR2/4–NF-κB–COX-2 inflammatory signaling in rats with Gardnerella vaginalis -associated bacterial vaginosis. (A) Relative protein abundance of Toll-like receptor 2 (TLR2), TLR4, phospho-NF-κB p65 at serine 536 (pS536-p65) and cyclooxygenase-2 (COX-2) in uterine tissues. Data are presented as the means ( X ¯ ) ± standard error of the mean (SEM); n = 3 independent uterine tissue samples per group. Statistical significance was determined using Student’s t-tests . Complete densitometric values are provided in Supplementary Table 11 . (B) Representative Western blot images. Protein levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Original uncropped Western blot images are provided in Supplementary Figure 1 .

    Article Snippet: After blocking with a rapid blocking buffer (Epizyme, Shanghai, China), the blots were incubated overnight at 4 °C with the following primary antibodies: TLR2 (Abcam, Cambridge, UK; dilution 1:1000), TLR4 (Proteintech, Hubei, China; dilution 1:1000), phospho-NF-κB p65 at serine 536 (pS536-p65; Proteintech; dilution 1:2000), cyclooxygenase-2 (COX-2; Proteintech; dilution 1:1000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Proteintech; dilution 1:10000).

    Techniques: Quantitative Proteomics, Tissue, Western Blot