anti cox2 (ABclonal Biotechnology)
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Anti Cox2, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cox2/MTCO2+Rabbit+mAb/pm42849804-72-42-44
Average 93 stars, based on 5 article reviews
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Incubation:Article Title: Phytohormone Strigolactams Overcomes Oxaliplatin Resistance in Colorectal Cancer by Blocking the Late-Stage Autophagy to Tip the Metabolic Balance toward Ferroptosis. Article Snippet: Primary and acquired oxaliplatin (OXA)-resistance remains a major challenge in colorectal cancer (CRC) therapy.. In this work, the type-II strigolactam SL 39 was identified as a promising lead compound against OXA-resistant HCT116R cells.. Co-treatment of SL 39 with OXA restored its sensitivity to HCT116R cells and inhibited xenograft tumor growth. Article Title: Astaxanthin suppress ferroptosis through the Akt1-FoxO3a signaling pathway to alleviates brain injury after intracerebral hemorrhage. Article Snippet: Following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Servicebio, Wuhan, China), proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, USA). .. Membranes were blocked with 5 % skim milk at room temperature for 2 h. Subsequently, membranes were incubated overnight at 4 ◦C with the following primary antibodies: GPX4 (67763-1-Ig; 1:2000; Proteintech Group, China), SLC7A11 (26864-1-AP; 1:2000; Proteintech Group, China), phosphorylated Akt1 (P-Akt1; 80462-1-RR; 1:4000; Proteintech Group, China), total Akt1 (T-Akt1; 80816-1-RR; 1:10000; Proteintech Group, China), phosphorylated FoxO3a (P-FoxO3a; 28755-1-AP; 1:1000; Proteintech Group, China), total FoxO3a (T-FoxO3a; 10849-1- AP; 1:5000; Proteintech Group, China), GAPDH (60004-1-AP; 1:100000; Proteintech Group, China), Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics. Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), Western Blot:Article Title: Manganese-loaded carbon nanoparticles ameliorate ANCA-associated vasculitis by inhibiting ferroptosis. Article Snippet: .. Western blotting (WB) was performed using specific primary antibodies against TFR1, manganese superoxide dismutase (MnSOD) (ABclonal, China), SDS Page:Article Title: Nicotinamide mononucleotide combined glucose counteracts iron overload induced mitochondrial dysfunction in mice under hypobaric hypoxia. Article Snippet: .. SDS-PAGE separated the proteins, transferred them onto a nitrocellulose membrane, and probed with specific antibodies against SLC7A11 (ABclonal, A13685), FSP1 (ABclonal, A12128), GPX4 (ProteinTech, 67763-1-Ig), COX1, (ABclonal, A7531), Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics. Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), Membrane:Article Title: Nicotinamide mononucleotide combined glucose counteracts iron overload induced mitochondrial dysfunction in mice under hypobaric hypoxia. Article Snippet: .. SDS-PAGE separated the proteins, transferred them onto a nitrocellulose membrane, and probed with specific antibodies against SLC7A11 (ABclonal, A13685), FSP1 (ABclonal, A12128), GPX4 (ProteinTech, 67763-1-Ig), COX1, (ABclonal, A7531), Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics. Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), Lysis:Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics. Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), Nucleic Acid Electrophoresis:Article Title: Icaritin alleviates UVB-induced skin damage by inhibiting ferroptosis via modulation of mitochondrial dynamics. Article Snippet: Cells were incubated with 5 μM C11-BODIPY 581/591 (Abclonal, RM02821) at 37◦C in the dark for 60 min, washed with PBS, stained with Hoechst dye solution, and imaged using a fluorescence microscope. .. Total proteins were lysed and extracted in lysis buffer, and quantified, SDS-PAGE gel electrophoresis, transferred to PVDF membrane, blocked for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies GPX4 (1:1500, Abclonal, A11243), Pyrolysis Gas Chromatography:Article Title: Periplocin and bufalin induce cardiotoxicity by regulating AMPK/SIRT1/PGC-1α pathway to inhibit energy metabolism and trigger autophagy. Article Snippet: Objective: Periplocin (PE) and bufalin (BU)—active ingredients of traditional Chinese medicine—exhibit diverse biological activities, including cardiotonic and antitumor effects.. However, their clinical application is limited by dose-dependent cardiotoxicity, the specific mechanisms of which remain unclear.. Methods: The cardiotoxicity of PE and BU was evaluated using cardiomyoblasts and zebrafish as models. Ubiquitin Proteomics:Article Title: Periplocin and bufalin induce cardiotoxicity by regulating AMPK/SIRT1/PGC-1α pathway to inhibit energy metabolism and trigger autophagy. Article Snippet: Objective: Periplocin (PE) and bufalin (BU)—active ingredients of traditional Chinese medicine—exhibit diverse biological activities, including cardiotonic and antitumor effects.. However, their clinical application is limited by dose-dependent cardiotoxicity, the specific mechanisms of which remain unclear.. Methods: The cardiotoxicity of PE and BU was evaluated using cardiomyoblasts and zebrafish as models. other:Article Title: A colon-targeted nanozyme-inulin hydrogel system for reactive oxygen species scavenging and gut microbiota modulation in inflammatory bowel disease therapy. Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article. |

